England's Frank Lampard has been ruled out of Euro 2012 with a thigh injury suffered in training on Wednesday.
The Football Association confirmed the news after the midfielder, 33, was examined by medical staff after a scan.
The midfielder suffered a serious injury when he collapsed clutching his thigh after running to retrieve a ball during training on Wednesday.
England manager Roy Hodgson has already lost Gareth Barry and he has a further concern that Scott Parker is only just back from an achilles problem.
Liverpool midfielder Jordan Henderson will be called into the squad, subject to agreement from Uefa's medical team.
Henderson is not a popular choice with fans after his poor first season since joining Liverpool in a £20million deal from Sunderland last summer.